Produced:Article Title: OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ACO2 Cell Signaling Technology Cat# 6571; RRID:AB_2797630 AIF Santa Cruz Biotechnology Cat# sc-13116; RRID:AB_626654 ALR Santa Cruz Biotechnology Cat# sc-134869; RRID:AB_10608344 ATPB Abcam Cat# ab5432; RRID:AB_304883 ATP5A Abcam Cat# ab14748; RRID:AB_301447 ATP5B Rabbit Serum In-house produced, a gift from Prof. P. Rehling ANT2 Cell Signaling Technology Cat# 14671; RRID:AB_2798562 COA7 Sigma-Aldrich Cat# HPA029926; RRID:AB_10602173 COX6A 3282.7 In-house produced, a gift from Prof. P. Rehling COX4 Cell Signaling Technology Cat# 4850; RRID:AB_2085424 CYCS Abcam Cat# ab133504; RRID:AB_2802115 DNAJC19 Proteintech Cat# 12096-1-AP; RRID:AB_2094914 FLAG tag Sigma-Aldrich Cat# F3165; RRID:AB_259529 FLAG tag Thermo Fisher Scientific Cat# PA1-984B; RRID:AB_347227 HA-Tag Cell Signaling Technology Cat# 3724 (also 3724S); RRID:AB_1549585 MRPL55 Proteintech Cat# 17679-1-AP; RRID:AB_2878427 MIC60 Abcam Cat# ab137057 Mitofusin-1 Cell Signaling Technology Cat# 14739; RRID:AB_2744531 OCIAD1 GeneTex Cat# GTX85234; RRID:AB_10734387 PHB1 Cell Signaling Technology Cat# 2426; RRID:AB_823689 PHB2 Cell Signaling Technology Cat# 14085; RRID:AB_2798387 TIMM21 Rabbit Serum In-house produced, a gift from Prof. P. Rehling TIMM22 Proteintech Cat# 14927-1-AP; RRID:AB_11183050 TIMM23 BD Biosciences Cat# 611222; RRID:AB_398754 TIMM17A Abcam Cat# ab192246 TIMM17B Thermo Fisher Scientific Cat# PA5-56054; RRID:AB_2648475 TIMM44 Proteintech Cat# 13859-1-AP; RRID:AB_2204679 TOMM20 Santa Cruz Biotechnology Cat# sc-11415; RRID:AB_2207533 TOMM40 Santa Cruz Biotechnology Cat# sc-365467; RRID:AB_10847086 TOMM70 Atlas Antibodies Cat# HPA048020; RRID:AB_2680234 Tubulin Santa Cruz Biotechnology Cat# sc-134239; RRID:AB_2210215 SAMM50 Abcam Cat# ab167430 SDHA Santa Cruz Biotechnology Cat# sc-166947; RRID:AB_10610526 SDHC Abcam Cat# ab155999; RRID:AB_2810989 UQCRC1 Sigma-Aldrich Cat# HPA002815; RRID:AB_1080486 VDAC1 Abcam Cat# ab15895; RRID:AB_2214787 YME1L Proteintech Cat# 11510-1-AP; RRID:AB_2217459 Chemicals, peptides, and recombinant proteins jetPRIME Polyplus transfection Polyplus 114 (Continued on next page) Cell Reports 43, 115038, December 24, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER TNT SP6 Quick Coupled Transcription/Translation System Promega L2080 [35S]Methionine PerkinElmer NEG009T005MC TMRM Thermo Fisher Scientific M20036 Digitonin Calbiochem 300410 Calibration Kit High Molecular Weight For Electrophoresis Cytvia GE17-0445-01 LipofectamineTM RNAiMAX Transfection Reagent Thermo Fisher Scientific 13778150 Mission siRNA universal negative control Sigma SIC001 FLAG Peptide Sigma F3290 PierceTM Anti-HA Agarose Thermo Fisher Scientific 26182 anti-FLAG M2 affinity gel Sigma A2220 TMT10plexTM Isobaric Label Reagent Thermo Fisher Scientific 90110 Critical commercial assays RNeasy Plus Mini Kit Qiagen 74134 SuperScriptTM IV First-Strand Synthesis System Thermo Fisher Scientific 18091050 Maxima First Strand cDNA Synthesis Kit Thermo Fisher Scientific K1671 SensiFASTTM SYBR Hi-ROX Kit Bioline BIO-92020 Deposited data The mass spectrometry data of OCIAD1FLAG affinity purification ProteomeXchange Consortium PXD043863 Experimental models: Cell lines HEK293 cell line American Type Culture Collection (ATCC) – HEK293 OCIAD1-KO cell line This Paper NA HEK293 TIMM17B WT/Del cell line This Paper NA HEK293T cell line Richter et al.201932 NA HEK293T-TIMM23FLAG cell line Richter et al.2019 32 NA HEK293T-TIMM21FLAG Richter et al.2019 32 NA Oligonucleotides OCIAD1-F (for cloning guide RNA in pX330 vector) 50CACC(G)TTTTCGAGAGCCGAATGCAG30 This Paper OCIAD1-R (for cloning guide RNA in pX330 vector) 50AAACCTGCATTCGGCTCTCGAAAA(C)30 This Paper TIMM17B-F (for cloning guide RNA in pX330 vector) 50CACC(G)TTCACTATGGGTGTCATCGG30 This Paper TIMM17B-R (for cloning guide RNA in pX330 vector) 50AAACCCGATGACACCCATAGTGAAC30 This Paper TIMM17A-F (for cloning guide RNA in pTNT vector) 50AAAACTCGAGGCCACCATGGA GGAGTACGCGCGAGAGC 30 This Paper TIMM17A-R (for cloning guide RNA in pTNT vector) 50AAAAGCGGCCGCCTACTGATA TTGTCGATAGTCT30 This Paper TIMM17B-F (for cloning guide RNA in pTNT vector) 50AAAACTCGAGGCCACCATGGA GGAGTACGCTCGGGAGC30 This Paper TIMM17B-R (for cloning guide RNA in pTNT vector) 50AAAAGCGGCCGCTCAGTGGTA CTGCTGATAGCTG30 This Paper OCIAD1 sense strand (siRNA Knockdown) 50GAGCCAAUUCCAUUCAGUU30 This Paper OCIAD1 anti-sense strand (siRNA Knockdown) 50ACUGAAUGGAAUUGGCUC30 This Paper (Continued on next page) 16 Cell Reports 43, 115038, December 24, 2024 Article ll OPEN ACCESS
FLAG-tag:Article Title: OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ACO2 Cell Signaling Technology Cat# 6571; RRID:AB_2797630 AIF Santa Cruz Biotechnology Cat# sc-13116; RRID:AB_626654 ALR Santa Cruz Biotechnology Cat# sc-134869; RRID:AB_10608344 ATPB Abcam Cat# ab5432; RRID:AB_304883 ATP5A Abcam Cat# ab14748; RRID:AB_301447 ATP5B Rabbit Serum In-house produced, a gift from Prof. P. Rehling ANT2 Cell Signaling Technology Cat# 14671; RRID:AB_2798562 COA7 Sigma-Aldrich Cat# HPA029926; RRID:AB_10602173 COX6A 3282.7 In-house produced, a gift from Prof. P. Rehling COX4 Cell Signaling Technology Cat# 4850; RRID:AB_2085424 CYCS Abcam Cat# ab133504; RRID:AB_2802115 DNAJC19 Proteintech Cat# 12096-1-AP; RRID:AB_2094914 FLAG tag Sigma-Aldrich Cat# F3165; RRID:AB_259529 FLAG tag Thermo Fisher Scientific Cat# PA1-984B; RRID:AB_347227 HA-Tag Cell Signaling Technology Cat# 3724 (also 3724S); RRID:AB_1549585 MRPL55 Proteintech Cat# 17679-1-AP; RRID:AB_2878427 MIC60 Abcam Cat# ab137057 Mitofusin-1 Cell Signaling Technology Cat# 14739; RRID:AB_2744531 OCIAD1 GeneTex Cat# GTX85234; RRID:AB_10734387 PHB1 Cell Signaling Technology Cat# 2426; RRID:AB_823689 PHB2 Cell Signaling Technology Cat# 14085; RRID:AB_2798387 TIMM21 Rabbit Serum In-house produced, a gift from Prof. P. Rehling TIMM22 Proteintech Cat# 14927-1-AP; RRID:AB_11183050 TIMM23 BD Biosciences Cat# 611222; RRID:AB_398754 TIMM17A Abcam Cat# ab192246 TIMM17B Thermo Fisher Scientific Cat# PA5-56054; RRID:AB_2648475 TIMM44 Proteintech Cat# 13859-1-AP; RRID:AB_2204679 TOMM20 Santa Cruz Biotechnology Cat# sc-11415; RRID:AB_2207533 TOMM40 Santa Cruz Biotechnology Cat# sc-365467; RRID:AB_10847086 TOMM70 Atlas Antibodies Cat# HPA048020; RRID:AB_2680234 Tubulin Santa Cruz Biotechnology Cat# sc-134239; RRID:AB_2210215 SAMM50 Abcam Cat# ab167430 SDHA Santa Cruz Biotechnology Cat# sc-166947; RRID:AB_10610526 SDHC Abcam Cat# ab155999; RRID:AB_2810989 UQCRC1 Sigma-Aldrich Cat# HPA002815; RRID:AB_1080486 VDAC1 Abcam Cat# ab15895; RRID:AB_2214787 YME1L Proteintech Cat# 11510-1-AP; RRID:AB_2217459 Chemicals, peptides, and recombinant proteins jetPRIME Polyplus transfection Polyplus 114 (Continued on next page) Cell Reports 43, 115038, December 24, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER TNT SP6 Quick Coupled Transcription/Translation System Promega L2080 [35S]Methionine PerkinElmer NEG009T005MC TMRM Thermo Fisher Scientific M20036 Digitonin Calbiochem 300410 Calibration Kit High Molecular Weight For Electrophoresis Cytvia GE17-0445-01 LipofectamineTM RNAiMAX Transfection Reagent Thermo Fisher Scientific 13778150 Mission siRNA universal negative control Sigma SIC001 FLAG Peptide Sigma F3290 PierceTM Anti-HA Agarose Thermo Fisher Scientific 26182 anti-FLAG M2 affinity gel Sigma A2220 TMT10plexTM Isobaric Label Reagent Thermo Fisher Scientific 90110 Critical commercial assays RNeasy Plus Mini Kit Qiagen 74134 SuperScriptTM IV First-Strand Synthesis System Thermo Fisher Scientific 18091050 Maxima First Strand cDNA Synthesis Kit Thermo Fisher Scientific K1671 SensiFASTTM SYBR Hi-ROX Kit Bioline BIO-92020 Deposited data The mass spectrometry data of OCIAD1FLAG affinity purification ProteomeXchange Consortium PXD043863 Experimental models: Cell lines HEK293 cell line American Type Culture Collection (ATCC) – HEK293 OCIAD1-KO cell line This Paper NA HEK293 TIMM17B WT/Del cell line This Paper NA HEK293T cell line Richter et al.201932 NA HEK293T-TIMM23FLAG cell line Richter et al.2019 32 NA HEK293T-TIMM21FLAG Richter et al.2019 32 NA Oligonucleotides OCIAD1-F (for cloning guide RNA in pX330 vector) 50CACC(G)TTTTCGAGAGCCGAATGCAG30 This Paper OCIAD1-R (for cloning guide RNA in pX330 vector) 50AAACCTGCATTCGGCTCTCGAAAA(C)30 This Paper TIMM17B-F (for cloning guide RNA in pX330 vector) 50CACC(G)TTCACTATGGGTGTCATCGG30 This Paper TIMM17B-R (for cloning guide RNA in pX330 vector) 50AAACCCGATGACACCCATAGTGAAC30 This Paper TIMM17A-F (for cloning guide RNA in pTNT vector) 50AAAACTCGAGGCCACCATGGA GGAGTACGCGCGAGAGC 30 This Paper TIMM17A-R (for cloning guide RNA in pTNT vector) 50AAAAGCGGCCGCCTACTGATA TTGTCGATAGTCT30 This Paper TIMM17B-F (for cloning guide RNA in pTNT vector) 50AAAACTCGAGGCCACCATGGA GGAGTACGCTCGGGAGC30 This Paper TIMM17B-R (for cloning guide RNA in pTNT vector) 50AAAAGCGGCCGCTCAGTGGTA CTGCTGATAGCTG30 This Paper OCIAD1 sense strand (siRNA Knockdown) 50GAGCCAAUUCCAUUCAGUU30 This Paper OCIAD1 anti-sense strand (siRNA Knockdown) 50ACUGAAUGGAAUUGGCUC30 This Paper (Continued on next page) 16 Cell Reports 43, 115038, December 24, 2024 Article ll OPEN ACCESS
Recombinant:Article Title: OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ACO2 Cell Signaling Technology Cat# 6571; RRID:AB_2797630 AIF Santa Cruz Biotechnology Cat# sc-13116; RRID:AB_626654 ALR Santa Cruz Biotechnology Cat# sc-134869; RRID:AB_10608344 ATPB Abcam Cat# ab5432; RRID:AB_304883 ATP5A Abcam Cat# ab14748; RRID:AB_301447 ATP5B Rabbit Serum In-house produced, a gift from Prof. P. Rehling ANT2 Cell Signaling Technology Cat# 14671; RRID:AB_2798562 COA7 Sigma-Aldrich Cat# HPA029926; RRID:AB_10602173 COX6A 3282.7 In-house produced, a gift from Prof. P. Rehling COX4 Cell Signaling Technology Cat# 4850; RRID:AB_2085424 CYCS Abcam Cat# ab133504; RRID:AB_2802115 DNAJC19 Proteintech Cat# 12096-1-AP; RRID:AB_2094914 FLAG tag Sigma-Aldrich Cat# F3165; RRID:AB_259529 FLAG tag Thermo Fisher Scientific Cat# PA1-984B; RRID:AB_347227 HA-Tag Cell Signaling Technology Cat# 3724 (also 3724S); RRID:AB_1549585 MRPL55 Proteintech Cat# 17679-1-AP; RRID:AB_2878427 MIC60 Abcam Cat# ab137057 Mitofusin-1 Cell Signaling Technology Cat# 14739; RRID:AB_2744531 OCIAD1 GeneTex Cat# GTX85234; RRID:AB_10734387 PHB1 Cell Signaling Technology Cat# 2426; RRID:AB_823689 PHB2 Cell Signaling Technology Cat# 14085; RRID:AB_2798387 TIMM21 Rabbit Serum In-house produced, a gift from Prof. P. Rehling TIMM22 Proteintech Cat# 14927-1-AP; RRID:AB_11183050 TIMM23 BD Biosciences Cat# 611222; RRID:AB_398754 TIMM17A Abcam Cat# ab192246 TIMM17B Thermo Fisher Scientific Cat# PA5-56054; RRID:AB_2648475 TIMM44 Proteintech Cat# 13859-1-AP; RRID:AB_2204679 TOMM20 Santa Cruz Biotechnology Cat# sc-11415; RRID:AB_2207533 TOMM40 Santa Cruz Biotechnology Cat# sc-365467; RRID:AB_10847086 TOMM70 Atlas Antibodies Cat# HPA048020; RRID:AB_2680234 Tubulin Santa Cruz Biotechnology Cat# sc-134239; RRID:AB_2210215 SAMM50 Abcam Cat# ab167430 SDHA Santa Cruz Biotechnology Cat# sc-166947; RRID:AB_10610526 SDHC Abcam Cat# ab155999; RRID:AB_2810989 UQCRC1 Sigma-Aldrich Cat# HPA002815; RRID:AB_1080486 VDAC1 Abcam Cat# ab15895; RRID:AB_2214787 YME1L Proteintech Cat# 11510-1-AP; RRID:AB_2217459 Chemicals, peptides, and recombinant proteins jetPRIME Polyplus transfection Polyplus 114 (Continued on next page) Cell Reports 43, 115038, December 24, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER TNT SP6 Quick Coupled Transcription/Translation System Promega L2080 [35S]Methionine PerkinElmer NEG009T005MC TMRM Thermo Fisher Scientific M20036 Digitonin Calbiochem 300410 Calibration Kit High Molecular Weight For Electrophoresis Cytvia GE17-0445-01 LipofectamineTM RNAiMAX Transfection Reagent Thermo Fisher Scientific 13778150 Mission siRNA universal negative control Sigma SIC001 FLAG Peptide Sigma F3290 PierceTM Anti-HA Agarose Thermo Fisher Scientific 26182 anti-FLAG M2 affinity gel Sigma A2220 TMT10plexTM Isobaric Label Reagent Thermo Fisher Scientific 90110 Critical commercial assays RNeasy Plus Mini Kit Qiagen 74134 SuperScriptTM IV First-Strand Synthesis System Thermo Fisher Scientific 18091050 Maxima First Strand cDNA Synthesis Kit Thermo Fisher Scientific K1671 SensiFASTTM SYBR Hi-ROX Kit Bioline BIO-92020 Deposited data The mass spectrometry data of OCIAD1FLAG affinity purification ProteomeXchange Consortium PXD043863 Experimental models: Cell lines HEK293 cell line American Type Culture Collection (ATCC) – HEK293 OCIAD1-KO cell line This Paper NA HEK293 TIMM17B WT/Del cell line This Paper NA HEK293T cell line Richter et al.201932 NA HEK293T-TIMM23FLAG cell line Richter et al.2019 32 NA HEK293T-TIMM21FLAG Richter et al.2019 32 NA Oligonucleotides OCIAD1-F (for cloning guide RNA in pX330 vector) 50CACC(G)TTTTCGAGAGCCGAATGCAG30 This Paper OCIAD1-R (for cloning guide RNA in pX330 vector) 50AAACCTGCATTCGGCTCTCGAAAA(C)30 This Paper TIMM17B-F (for cloning guide RNA in pX330 vector) 50CACC(G)TTCACTATGGGTGTCATCGG30 This Paper TIMM17B-R (for cloning guide RNA in pX330 vector) 50AAACCCGATGACACCCATAGTGAAC30 This Paper TIMM17A-F (for cloning guide RNA in pTNT vector) 50AAAACTCGAGGCCACCATGGA GGAGTACGCGCGAGAGC 30 This Paper TIMM17A-R (for cloning guide RNA in pTNT vector) 50AAAAGCGGCCGCCTACTGATA TTGTCGATAGTCT30 This Paper TIMM17B-F (for cloning guide RNA in pTNT vector) 50AAAACTCGAGGCCACCATGGA GGAGTACGCTCGGGAGC30 This Paper TIMM17B-R (for cloning guide RNA in pTNT vector) 50AAAAGCGGCCGCTCAGTGGTA CTGCTGATAGCTG30 This Paper OCIAD1 sense strand (siRNA Knockdown) 50GAGCCAAUUCCAUUCAGUU30 This Paper OCIAD1 anti-sense strand (siRNA Knockdown) 50ACUGAAUGGAAUUGGCUC30 This Paper (Continued on next page) 16 Cell Reports 43, 115038, December 24, 2024 Article ll OPEN ACCESS
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